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anti couptfii  (R&D Systems)


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    Structured Review

    R&D Systems anti couptfii
    Anti Couptfii, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+couptfii/Human+COUP-TF+II%2FNR2F2+Antibody/pm41481707-418-10-12
    Average 94 stars, based on 80 article reviews
    anti couptfii - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Intergenerational effects on fertility in male and female mice after chronic exposure to environmental doses of NSAIDs and 17α-ethinylestradiol mixtures.
    Article Snippet: Non-steroidal anti-inflammatory drugs (NSAIDs) and 17α-ethinylestradiol (EE2) are extensively used in human and veterinary medicine.. Due to their partial removal by wastewater treatment plants, they are frequent environmental contaminants, particularly in drinking water.. Here, we investigated the adverse outcomes of chronic exposure to mixtures of NSAIDs (ibuprofen, 2hydroxy-ibuprofen, diclofenac) and EE2 at two environmentally relevant doses in drinking water, on the reproductive organ development and fertility in F1-exposed male and female mice and in their F2 offspring.

    Incubation:

    Article Title: Intergenerational effects on fertility in male and female mice after chronic exposure to environmental doses of NSAIDs and 17α-ethinylestradiol mixtures.
    Article Snippet: Non-steroidal anti-inflammatory drugs (NSAIDs) and 17α-ethinylestradiol (EE2) are extensively used in human and veterinary medicine.. Due to their partial removal by wastewater treatment plants, they are frequent environmental contaminants, particularly in drinking water.. Here, we investigated the adverse outcomes of chronic exposure to mixtures of NSAIDs (ibuprofen, 2hydroxy-ibuprofen, diclofenac) and EE2 at two environmentally relevant doses in drinking water, on the reproductive organ development and fertility in F1-exposed male and female mice and in their F2 offspring.

    Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation.
    Article Snippet: .. Tissues were incubated overnight at 4°C in blocking buffer containing anti–COUPTFII (1:200, R&D Systems, catalog no. PP- H7147- 00), anti- FOXL2 (1:200, Novus Biological, catalog no. NB100- 1277), and anti- LEF1 (1:200, catalog no. PA5- 119910) antibodies. .. The next day, tissues were washed three times for 1 hour each in blocking buffer at 4°C, followed by incubation with donkey anti- mouse (1:200, Invitrogen, catalog no. A10037), donkey anti- goat (1:200, Invitrogen, catalog no. A21447), and donkey anti- rabbit (1:200, Invitrogen, catalog no. A21206) secondary antibodies diluted in blocking buffer incubation for 1 hour at room temperature.

    Article Title: Cocktails of NSAIDs and 17α Ethinylestradiol at Environmentally Relevant Doses in Drinking Water Alter Puberty Onset in Mice Intergenerationally.
    Article Snippet: .. Sections were then blocked with 10% donkey serum (Sigma-Aldrich) or 1/1000 anti-goat IgG (Thermofisher, Waltham, MA, USA), and incubated (4 ◦C, overnight) with the following primary antibodies: monoclonal mouse anti-PCNA (Sigma-Aldrich P8825, 1/500), anti-phospho-gH2Ax (Ser139 clone JBW301, Sigma Aldrich 05-636, 1/500), anti-COUPTFII (RD systems PP-H7147-00, 1/400), a-tubulin (Sigma Aldrich T9026, 1/1000), monoclonal rat anti-phosphorylated-Histone H3 (Ser10, Sigma Aldrich H9908, 1/400), anti-Tra98 (Abcam ab82527, 1/400), polyclonal guinea pig anti-PADI6 (1/500), polyclonal goat anti-Sox9 (RD systems AF3075, 1/300), polyclonal rabbit anti-FoxL2 (homemade [149], 1/300), anti-AMH (gift from N DiClemente, 1/300), anti-Lin28a (ProteinTech 11724, 1/400), anti-connexin-43 (Sigma Aldrich C6219, 1/500), and anti-a Smooth muscle actin (SMA) (Abcam ab124964, 1/500). .. Sections were then incubated with donkey or goat secondary antibodies conjugated with Alexa Fluor 555, 488, or 649 (Thermofisher, 1/1000) at room temperature for 30 min, followed by Hoechst staining.

    Blocking Assay:

    Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation.
    Article Snippet: .. Tissues were incubated overnight at 4°C in blocking buffer containing anti–COUPTFII (1:200, R&D Systems, catalog no. PP- H7147- 00), anti- FOXL2 (1:200, Novus Biological, catalog no. NB100- 1277), and anti- LEF1 (1:200, catalog no. PA5- 119910) antibodies. .. The next day, tissues were washed three times for 1 hour each in blocking buffer at 4°C, followed by incubation with donkey anti- mouse (1:200, Invitrogen, catalog no. A10037), donkey anti- goat (1:200, Invitrogen, catalog no. A21447), and donkey anti- rabbit (1:200, Invitrogen, catalog no. A21206) secondary antibodies diluted in blocking buffer incubation for 1 hour at room temperature.



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